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CD44 抗体

The 小鼠 单克隆 anti-CD44 antibody (Clone GT462) (ABIN7464320) specifically detects CD44 in FACS, WB, IHC (p) 和 IP. The antibody is reactive with 人 samples.
产品编号 ABIN7464320
发货至: 中国
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Quick Overview for CD44 抗体 (ABIN7464320)

抗原

See all CD44 抗体
CD44

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD44 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

质量等级

KO Validated

克隆位点

GT462
  • 特异性

    IP/MS validation was supported by references (PMID:30377425)

    交叉反应

    纯化方法

    Affinity purified by Protein G.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human CD44. The exact sequence is proprietary.

    亚型

    IgG2b
  • 应用备注

    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: A431 , MDA-MB-231(24 μg/ml Tunicamycin treatment for 16 hr) , HeLa(12 μg/ml Tunicamycin treatment for 48 hr)

    Validation: IP/MS, KO/KD, Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.7 mg/mL

    缓冲液

    PBS, No Preservative

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    CD44

    别名

    CD44 molecule (Indian blood group)

    背景

    CD44 Molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1,The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

    分子量

    82 kDa

    基因ID

    960

    UniProt

    P16070

    途径

    Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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