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beta Actin 抗体 (C-Term)

This anti-beta Actin antibody is a 小鼠 单克隆 antibody detecting beta Actin in WB, IF, IHC (p), ICC 和 IP. Suitable for 人.
产品编号 ABIN7464288
发货至: 中国

Quick Overview for beta Actin 抗体 (C-Term) (ABIN7464288)

抗原

See all beta Actin (ACTB) 抗体
beta Actin (ACTB) (Actin, beta (ACTB))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This beta Actin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

质量等级

KO Validated

克隆位点

GT5512
  • 抗原表位

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    C-Term

    特异性

    IP/MS validation was supported by references (PMID:30377426)

    交叉反应

    Cat, 犬, Drosophila melanogaster, 山羊, 人, 猴, 小鼠, 兔, 大鼠, Saccharomyces cerevisiae, 非洲爪蟾, 斑马鱼

    纯化方法

    Affinity purified by Protein G.

    免疫原

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

    亚型

    IgG1
  • 应用备注

    WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: 30 hpf zebrafish , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , rabbit blood , goat blood , MDA-MB-231

    Validation: IP/MS, KO/KD, Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, No Preservative

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    beta Actin (ACTB) (Actin, beta (ACTB))

    别名

    actin beta

    背景

    Actin beta , BRWS1 , PS1TP5BP1,This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

    分子量

    42 kDa

    基因ID

    60

    UniProt

    P60709

    途径

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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