beta Actin 抗体 (C-Term)
Quick Overview for beta Actin 抗体 (C-Term) (ABIN7464220)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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特异性
- IP/MS validation was supported by references (PMID:30377395)
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交叉反应
- C. elegans, Candida albicans, Cat, 小鸡, 犬, Drosophila melanogaster, E. coli, Hamster, 马, 人, 猴, 小鼠, Onchocerca, Oryza sativa, Pig, 植物, 大鼠, Saccharomyces cerevisiae, 绵羊, Tree shrew, 非洲爪蟾
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: 293T , A431 , Jurkat , Raji , mouse kidney , mouse lung , mouse testis , Candida albicans (sc5314) , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , Rat-2 , NTH-3T3 , mouse brain , PC-12 , Rat brain
Validation: IP/MS, KO/KD, Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.4 mg/mL
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缓冲液
- 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- actin beta
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背景
- Actin beta , BRWS1 , PS1TP5BP1,This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
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分子量
- 42 kDa
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基因ID
- 60
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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