RPA2 抗体 (C-Term)
Quick Overview for RPA2 抗体 (C-Term) (ABIN7454599)
抗原
See all RPA2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Rabbit anti-RPA32 Antibody, Affinity Purified
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纯化方法
- Affinity Purified
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免疫原
- between AA 225 and C-term
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亚型
- IgG
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应用备注
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IHC: 1:2,000 to 1:10,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
IP: 2 - 10 μg/mg lysate
WB: 1:2,000 - 1:10,000
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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别名
- RPA32
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背景
- Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA2, the 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
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基因ID
- 6118
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NCBI登录号
- NP_002937
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UniProt
- P15927
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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