Recombinant RPA2 抗体 (pSer4, pSer8)
Quick Overview for Recombinant RPA2 抗体 (pSer4, pSer8) (ABIN7453379)
抗原
See all RPA2 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer4, pSer8
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原理
- Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1]
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免疫原
- surrounding serine 4/8
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亚型
- IgG
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应用备注
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ICC-IF: 1:1,000 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
IP: 6 μL/1.0 mg lysate
ICC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
WB: 17:40:00
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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别名
- RPA32
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背景
- Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA2, the 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
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基因ID
- 6118
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NCBI登录号
- NP_002937
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UniProt
- P15927
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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