Histone 3 抗体 (pSer10)
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Quick Overview for Histone 3 抗体 (pSer10) (ABIN7449558)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer10
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原理
- Rabbit anti-Phospho Histone H3 (S10) Antibody, Affinity Purified
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预测反应
- Rat,D. melanogaster,Zebrafish,X. laevis,X. tropicalis,Chicken,Bovine,Rabbit,Pig,Orangutan
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纯化方法
- Affinity Purified
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免疫原
- surrounding serine 10
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亚型
- IgG
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应用备注
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ICC-IF: 1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining.
IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
WB: 1:5,000 - 1:10,000
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
- Background: Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.
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基因ID
- 8350
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NCBI登录号
- NP_003520
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UniProt
- P68431
抗原
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