MRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed, mOrange
纯化方法
Produced in: E.coli
免疫原
Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among others family members. Look at our specificity chart in the resource section.
RFP
适用: Discosoma
WB, ELISA, IF
宿主: 兔
Polyclonal
HRP
应用备注
Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconsitute with 250 µL 50% glycerol for storage at -80 °C
缓冲液
sdAbs are lyophilized from PBS pH 7.4.
储存条件
4 °C/-20 °C
储存方法
Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.