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Recombinant GFP 抗体

This anti-GFP antibody is a Alpaca 单克隆 antibody detecting GFP in IF, ICC, FACS, DNA Mic, MA 和 mIF. Suitable for Aequorea victoria 和 Aequorea aequorea.
产品编号 ABIN7272887
发货至: 中国

Quick Overview for Recombinant GFP 抗体 (ABIN7272887)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

抗体类型

Recombinant Antibody

片段

single-domain Antibody (sdAb)

适用

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Aequorea victoria, Aequorea aequorea

宿主

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Alpaca

克隆类型

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单克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), DNA Microscopy (DNA Mic), Multiplex Assay (MA), Multiplex Immunofluorescence (mIF)

克隆位点

1H1
  • Expression System

    E.coli

    特异性

    GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus, mClover3 and Citrine

    纯化方法

    Produced in: E.coli

    免疫原

    Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria
  • 应用备注

    Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconsitute with 250 µL pure water for immidate use

    缓冲液

    sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.

    储存条件

    4 °C/-20 °C

    储存方法

    Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    A breakthrough in the field of biology and bioluminescence started in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants here.
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