TMEM119 抗体 (Extracellular) (APC)
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Quick Overview for TMEM119 抗体 (Extracellular) (APC) (ABIN7237921)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 53-67, Extracellular
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原理
- A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
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特异性
- Extracellular, N-terminus.
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- This antibody will not recognize human TMEM119.
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预测反应
- Rat - 12 out of 15 amino acid residues identical This antibody will not recognize human TMEM119
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产品特性
- Extracellular, N-terminus
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119
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亚型
- IgG
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anti-Transmembrane Protein 119 (TMEM119) (AA 53-67), (Extracellular) antibody
TMEM119 适用: 小鼠 FACS, WB 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 119 (TMEM119) (AA 53-67), (Extracellular) antibody (FITC)TMEM119 适用: 小鼠 FACS 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582043)
Blocking Peptide: (ABIN7237517)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- TMEM119 (Transmembrane Protein 119 (TMEM119))
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别名
- TMEM119
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背景
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Transmembrane Protein 119, Osteoblast induction factor, OBIF,Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytes/macrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.
Alternative names: Transmembrane Protein 119, Osteoblast induction factor, OBIF -
基因ID
- 231633
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NCBI登录号
- NM_181724
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UniProt
- Q8R138
抗原
-
