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TMEM119 抗体 (Extracellular) (APC)

This anti-TMEM119 antibody is a 兔 多克隆 antibody detecting TMEM119 in FACS 和 LCI. Suitable for 小鼠.
产品编号 ABIN7237921
发货至: 中国

Quick Overview for TMEM119 抗体 (Extracellular) (APC) (ABIN7237921)

抗原

TMEM119 (Transmembrane Protein 119 (TMEM119))

适用

  • 4
  • 2
  • 1
小鼠

宿主

  • 5
  • 3
  • 1

克隆类型

  • 4
  • 3
  • 1
多克隆

标记

  • 7
  • 1
This TMEM119 antibody is conjugated to APC

应用范围

  • 6
  • 6
  • 3
  • 3
  • 1
Flow Cytometry (FACS), Live Cell Imaging (LCI)
  • 抗原表位

    • 2
    • 2
    • 1
    • 1
    AA 53-67, Extracellular

    原理

    A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)

    特异性

    Extracellular, N-terminus.

    交叉反应

    小鼠, 大鼠

    交叉反应 (详细)

    This antibody will not recognize human TMEM119.

    预测反应

    Rat - 12 out of 15 amino acid residues identical This antibody will not recognize human TMEM119

    产品特性

    Extracellular, N-terminus

    纯化方法

    Affinity purified on immobilized antigen.

    免疫原

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119

    亚型

    IgG
  • 应用备注

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: N/A

    说明

    Negative Control: (ABIN7582043)

    Blocking Peptide: (ABIN7237517)

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • 抗原

    TMEM119 (Transmembrane Protein 119 (TMEM119))

    别名

    TMEM119

    背景

    Transmembrane Protein 119, Osteoblast induction factor, OBIF,Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytes/macrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

    Alternative names: Transmembrane Protein 119, Osteoblast induction factor, OBIF

    基因ID

    231633

    NCBI登录号

    NM_181724

    UniProt

    Q8R138
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