TMEM119 抗体 (Extracellular, N-Term) (FITC)
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Quick Overview for TMEM119 抗体 (Extracellular, N-Term) (FITC) (ABIN7043860)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 53-67, Extracellular, N-Term
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原理
- A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye FITC
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- This antibody will not recognize human TMEM119
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预测反应
- Rat - 12 out of 15 amino acid residues identical This antibody will not recognize human TMEM119
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产品特性
- Anti-TMEM119 (extracellular) Antibody (ABIN7043859, ABIN7044740 and ABIN7044741) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples. \nAnti-TMEM119 (extracellular)-FITC Antibody (ABIN7043859, ABIN7044740 and ABIN7044741)-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119
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亚型
- IgG
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anti-Transmembrane Protein 119 (TMEM119) (AA 53-67), (Extracellular), (N-Term) antibody
TMEM119 适用: 小鼠 FACS, WB 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 119 (TMEM119) (AA 53-67), (Extracellular), (N-Term) antibody (APC)TMEM119 适用: 小鼠 FACS, LCI 宿主: 兔 Polyclonal APC
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应用备注
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WB: The optimal concentration should be determined by the user
FC: 2.5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7582044)
Blocking Peptide: (ABIN7237517)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- TMEM119 (Transmembrane Protein 119 (TMEM119))
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别名
- TMEM119
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背景
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Synonyms: Transmembrane Protein 119, Osteoblast induction factor, OBIF
Description: Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.
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基因ID
- 231633
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NCBI登录号
- NM_181724
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UniProt
- Q8R138
抗原
-
