GPR18 抗体 (Extracellular, N-Term)
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Quick Overview for GPR18 抗体 (Extracellular, N-Term) (ABIN7237662)
抗原
See all GPR18 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 7-22, Extracellular, N-Term
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原理
- A Rabbit Polyclonal antibody to GPR18 (extracellular)
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - 13 out of 16 amino acid residues identical Not recommended for human samples
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产品特性
- Extracellular, N-terminus
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)HNQLDLSNGSHPEEYK, corresponding to amino acid residues 7 - 22 of mouse GPR18
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亚型
- IgG
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anti-G Protein-Coupled Receptor 18 (GPR18) (AA 290-331) antibody
GPR18 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (Cytoplasmic Domain) antibodyGPR18 适用: 人, 猴, Bat, 马, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (AA 1-50) antibodyGPR18 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (Cytoplasmic Domain) antibodyGPR18 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (Extracellular Domain) antibodyGPR18 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (Internal Region) antibodyGPR18 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (Extracellular Domain) antibodyGPR18 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (AA 144-193) antibodyGPR18 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (AA 191-240) antibodyGPR18 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 18 (GPR18) (AA 131-180) antibodyGPR18 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: 5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: 1:1000
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235388)
Blocking Peptide: (ABIN7235388)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- GPR18 (G Protein-Coupled Receptor 18 (GPR18))
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别名
- GPR18
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背景
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Synonyms: G-Protein Coupled Receptor 18, N-Arachidonyl Glycine Receptor, NAGly Receptor, GPCRW
Description: G protein coupled receptors (GPCRs) comprise a large superfamily of receptors characterized by a seven transmembrane domain structure and an ability to activate intracellular transducer G proteins. Over 800 GPCRs in five main families have been identified in eukaryotes on the basis of genomic sequence analysis 1-2. These receptors can be activated by hormones, neurotransmitters, odorants, light, or pheromones. GPCRs mediate the signaling of extracellular stimuli to intracellular responses via activation of heterotrimeric G proteins and their subsequent interaction with effector proteins. GPCRs have been implicated in a number of physiological functions including development of anxiety, behavior, homeostasis, cognition, appetite, and drug addiction 3. The GPCR family contains a diverse group of cell-surface mediators of signal transduction, its size exceeding other cell-surface protein receptors including the tyrosine kinase receptors, guanylyl cyclase receptors and ligand-gated ion channels 4. The murine homolog of GPR18 was first isolated in 1996 5. A potential agonist for this orphan receptor was proposed only in 2006 when N-arachidonylglycine (NAGly) was shown to increase calcium concentration and inhibit forskolin induced cAMP production in CHO cells in a pertussis sensitive manner 6. GPR18 shares low sequence homology with the cannabinoid receptors CB1R and CB2R (~13 % and 8 %) and moderate identity with the putative cannabinoid receptor GPR55 (21 %). Nonetheless, it has been related to the endocannabinoid system since a range of endogenous, phytogenic and synthetic cannabinoids have shown to modulate GPR18 7. GPR18 was shown to be constitutively active in melanomas, acting as an apoptosis inhibitor 8, while playing a reverse role in macrophage apoptosis, that is, signaling for cell death9. Both of these roles were mediated via Gαi pathways. Work on alternate cannabinoid compounds as potential agonist showed differential activation of Gαq / Gαi signaling suggesting biased agonism in pathway selection10.
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基因ID
- 110168
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NCBI登录号
- NM_001098200
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UniProt
- Q8K1Z6
抗原
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