GIT1 抗体 (C-Term)
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Quick Overview for GIT1 抗体 (C-Term) (ABIN7233038)
抗原
See all GIT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 375-770, C-Term
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原理
- Anti-GIT1 Mouse Monoclonal Antibody
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特异性
- This antibody recognizes human, mouse and rat GIT1. It does not cross-react with GIT2.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified by Protein G affinity chromatography.
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免疫原
- Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).
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亚型
- IgG1
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应用备注
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Immunoblotting: use at 1 μg/mL. Predicted molecular weight is ~90 kDa.
Positive control: rat brain lysate.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Dilute in PBS or medium that is identical to that used in the assay system.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- This antibody is stable for at least one (1) year at -20°C.
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- GIT1
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别名
- Git1
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背景
- ARF GTPase-activating protein GIT1,G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cyto- plasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.
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UniProt
- Q9Z272
抗原
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