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GIT1 抗体 (C-Term)

This anti-GIT1 antibody is a 小鼠 单克隆 antibody detecting GIT1 in WB, IP 和 IF/ICC. Suitable for 大鼠.
产品编号 ABIN7233038
发货至: 中国

Quick Overview for GIT1 抗体 (C-Term) (ABIN7233038)

抗原

See all GIT1 抗体
GIT1

适用

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  • 54
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  • 2
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  • 1
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  • 1
大鼠

宿主

  • 106
  • 20
小鼠

克隆类型

  • 97
  • 29
单克隆

标记

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  • 1
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This GIT1 antibody is un-conjugated

应用范围

  • 93
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Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

克隆位点

S39B-8
  • 抗原表位

    • 16
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    AA 375-770, C-Term

    原理

    Anti-GIT1 Mouse Monoclonal Antibody

    特异性

    This antibody recognizes human, mouse and rat GIT1. It does not cross-react with GIT2.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified by Protein G affinity chromatography.

    免疫原

    Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).

    亚型

    IgG1
  • 应用备注

    Immunoblotting: use at 1 μg/mL. Predicted molecular weight is ~90 kDa.
    Positive control: rat brain lysate.
    These are recommended concentrations.
    Endusers should determine optimal concentrations for their applications.

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Dilute in PBS or medium that is identical to that used in the assay system.

    浓度

    1.0 mg/mL

    缓冲液

    PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    This antibody is stable for at least one (1) year at -20°C.
  • 抗原

    GIT1

    别名

    Git1

    背景

    ARF GTPase-activating protein GIT1,G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cyto- plasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.

    UniProt

    Q9Z272
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