Crk 抗体
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北京 101111
Quick Overview for Crk 抗体 (ABIN7214340)
抗原
See all Crk (CRK) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Crk II Polyclonal Antibody
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特异性
- Crk II Polyclonal Antibody detects endogenous levels of Crk II protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Crk II around the non-phosphorylation site of Y221
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))
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别名
- Crk II
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背景
- Rabbit Anti-Crk II Polyclonal Antibody,CRK, Adapter molecule crk, Proto-oncogene c-Crk, p38,CRK encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.,Adapter molecule crk
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分子量
- observerd band 40kDa
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基因ID
- 1398
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UniProt
- P46108
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途径
- Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
抗原
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