MED14 抗体 (AA 670-750)
Quick Overview for MED14 抗体 (AA 670-750) (ABIN7225920)
抗原
See all MED14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 670-750
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原理
- CRSP150 Polyclonal Antibody
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特异性
- CRSP150 Polyclonal Antibody detects endogenous levels of CRSP150 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human CRSP150 at AA range: 670-750
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亚型
- IgG
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anti-Mediator Complex Subunit 14 (MED14) (Internal Region) antibody
MED14 适用: 人, 小鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (AA 586-615) antibodyMED14 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB20179 unconjugated
anti-Mediator Complex Subunit 14 (MED14) antibodyMED14 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (AA 575-624) antibodyMED14 适用: 人, 小鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (AA 701-750) antibodyMED14 适用: 人, 小鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (Middle Region) antibodyMED14 适用: 人, 小鼠, 兔 WB, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- MED14 (Mediator Complex Subunit 14 (MED14))
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别名
- CRSP150
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背景
- Rabbit Anti-CRSP150 Polyclonal Antibody,MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170, Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRS,The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED14 (mediator complex subunit 14) is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. MED14 is known to escape chromosome X-inactivation.,Mediator of RNA polymerase II transcription subunit 14
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基因ID
- 9282
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UniProt
- O60244
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
抗原
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