HNF4A 抗体 (pSer313)
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Quick Overview for HNF4A 抗体 (pSer313) (ABIN7213334)
抗原
See all HNF4A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer313
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原理
- Rabbit Anti-HNF4-α (phospho Ser313) Polyclonal Antibody
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特异性
- Phospho-HNF4-α (S313) Polyclonal Antibody detects endogenous levels of HNF4-α protein only when phosphorylated at S313.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized peptide derived from human HNF4-alpha Phospho-Ser313
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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有效期
- 12 months
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- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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别名
- HNF4-alpha
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背景
- HNF4A, HNF4, NR2A1, TCF14, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4Hepatocyte nuclear factor 4 alpha encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. Hepatocyte nuclear factor 4 alpha controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of HNF4A results in multiple transcript variants encoding several different isoforms.
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分子量
- 52kD
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基因ID
- 3172
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UniProt
- P41235
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途径
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
抗原
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