H2AFX 抗体 (pSer139)
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Quick Overview for H2AFX 抗体 (pSer139) (ABIN7212984)
抗原
See all H2AFX 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer139
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原理
- Histone H2A.X (phospho Ser139) Polyclonal Antibody
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特异性
- Phospho-Histone H2A.X (S139) Polyclonal Antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at S139.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Histone H2A.X Phospho-Ser139
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- H2AFX (H2A Histone Family, Member X (H2AFX))
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别名
- Histone H2A.X
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背景
- Rabbit Anti-Histone H2A.X (phospho Ser139) Polyclonal Antibody,H2AFX, H2AX, Histone H2A.x, H2a/x,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H2AFX encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.,Histone H2A.x
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分子量
- observerd band 15kDa
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基因ID
- 3014
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UniProt
- P16104
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途径
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原
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