RAD18 抗体 (AA 270-350)
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Quick Overview for RAD18 抗体 (AA 270-350) (ABIN7216744)
抗原
See all RAD18 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 270-350
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原理
- Rad18 Polyclonal Antibody
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特异性
- Rad18 Polyclonal Antibody detects endogenous levels of Rad18 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human Rad18 at AA range: 270-350
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
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别名
- Rad18
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背景
- Rabbit Anti-Rad18 Polyclonal Antibody,RAD18, RNF73, E3 ubiquitin-protein ligase RAD18, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73,RAD18, E3 ubiquitin protein ligase encoded by RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18, E3 ubiquitin protein ligase is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.,E3 ubiquitin-protein ligase RAD18
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分子量
- observerd band 56kDa
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基因ID
- 56852
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UniProt
- Q9NS91
抗原
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