Cullin 1 抗体 (AA 700-780)
Quick Overview for Cullin 1 抗体 (AA 700-780) (ABIN7214378)
抗原
See all Cullin 1 (CUL1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 700-780
-
原理
- CUL-1 Polyclonal Antibody
-
特异性
- CUL-1 Polyclonal Antibody detects endogenous levels of CUL-1 protein.
-
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
-
免疫原
- Synthesized peptide derived from the C-terminal region of human CUL-1 at AA range: 700-780
-
亚型
- IgG
-
-
anti-Cullin 1 (CUL1) (AA 500-776) antibody
CUL1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyKD Validated CUL1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 23GB1555 unconjugated Recombinant Antibody
anti-Cullin 1 (CUL1) antibodyCUL1 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 适用: 人, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-250) antibodyCUL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-776) antibodyCUL1 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 适用: 人, 小鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 适用: 人 WB, IHC, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
-
-
应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
-
说明
-
Primary Antibody
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
-
-
- Cullin 1 (CUL1)
-
别名
- CUL-1
-
背景
- Rabbit Anti-CUL-1 Polyclonal Antibody,CUL1, Cullin-1, CUL-1,Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.,Cullin-1
-
分子量
- observerd band 90kDa
-
基因ID
- 8454
-
UniProt
- Q13616
-
途径
- Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size
抗原
-
