Recombinant CD25 抗体
Quick Overview for Recombinant CD25 抗体 (ABIN7200649)
抗原
See all CD25 (IL2RA) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
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原理
- In vivo Grade Recombinant Anti-mouse CD25 Mouse IgG2c Kappa Monoclonal Antibody
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产品特性
- The in vivo grade recombinant anti-mouse CD25 monoclonal antibodies are based on the rat monoclonal antibody anti-CD25 (mouse) with the clone number of PC61, PC61.5 or PC61.5.3. The rat IgG1 lamda monoclonal antibody has been validated and reported for use in immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. In vivo depletion of mouse CD25(+)CD4(+)FoxP3(+) Treg cells using the rat PC61 monoclonal antibody is widely used to characterize the Treg function. The PC61 monoclonal antibody specifically binds to an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by the 3C7 and 7D4 monoclonal antibodies. By doing so, the antibody blocks binding of IL-2 to CD25.
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纯化方法
- Protein A affinity column
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纯度
- > 95% by SDS-PAGE under reducing conditions and HPLC.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1 EU per 1 mg of the protein by the LAL method.
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亚型
- IgG2c, kappa
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anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody
IL2RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B5D12 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibodyIL2RA 适用: 人 WB, ELISA, ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibodyIL2RA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibodyIL2RA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IL2RA-2395 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 PerCP
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-235) antibodyIL2RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4
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注意事项
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
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有效期
- 3 months
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- CD25
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原
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