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CD44 抗体 (AA 21-220)

Name: CD44 抗体 (AA 21-220)
Brand: antibodies-online
SKU: ABIN7147160
Availability: OutOfStock

This anti-CD44 antibody is a 小鼠单克隆 antibody detecting CD44 in FACS, WB, IHC, ELISA 和 IF. Suitable for 人.

Western Blot Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate All lanes: CD44 antibody at 1:1500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa Observed band size: 95 kDa (CD44 抗体 (AA 21-220))

Overlay histogram showing Hela cells stained with ABIN7147160 (red line) at 1:200. (CD44 抗体 (AA 21-220))

FACS

Western Blot Positive WB detected in: A549 whole cell lysate All lanes: CD44 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa Observed band size: 95 kDa (CD44 抗体 (AA 21-220))

IHC image of ABIN7147160 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (CD44 抗体 (AA 21-220))

IHC

IHC image of ABIN7147160 diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. (CD44 抗体 (AA 21-220))

IHC

IHC image of ABIN7147160 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. (CD44 抗体 (AA 21-220))

IHC

Immunofluorescence staining of Hela cells with ABIN7147160 at 1:100, counter-stained with DAPI. (CD44 抗体 (AA 21-220))

Immunofluorescence staining of MCF-7 cells with ABIN7147160 at 1:100, counter-stained with DAPI. (CD44 抗体 (AA 21-220))

8 images

产品编号 ABIN7147160

发货至: 中国

Datasheet as PDF

Quick Overview for CD44 抗体 (AA 21-220) (ABIN7147160)

抗原

See all CD44 抗体

CD44

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This CD44 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

克隆位点

6G2D2

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抗原表位
16

16

16

11

9

5

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 21-220

交叉反应

人

纯化方法

Protein G purified

免疫原

Recombinant Human CD44 antigen protein (21-220AA)

亚型

IgG1
anti-CD44 (CD44) antibody
CD44 适用: 人, 小鼠 FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8E2F3 unconjugated

anti-CD44 (CD44) antibody
KO Validated CD44 适用: 人 WB, IF, IHC (p), IP, IHC (fro), ICC, LCI 宿主: 兔 Polyclonal unconjugated

anti-CD44 (CD44) antibody
CD44 适用: 人 FACS, WB, IHC, ELISA, IHC (p), IP, Inhibition 宿主: 小鼠 Monoclonal MEM-85 unconjugated

anti-CD44 (CD44) antibody
CD44 适用: 人, 犬, Pig FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-263 unconjugated

anti-CD44 (CD44) (AA 148-247) antibody
CD44 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD44 (CD44) (AA 36-194) antibody
CD44 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7F4F1 unconjugated

anti-CD44 (CD44) (AA 21-259) antibody
CD44 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, ELISA, IF, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-CD44 (CD44) (AA 1-361) antibody
CD44 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated

anti-CD44 (CD44) antibody
CD44 适用: 人, Baboon, Green Monkey FACS, WB, IHC, IF, Func, StM 宿主: 小鼠 Monoclonal 156-3C11 unconjugated

anti-CD44 (CD44) (AA 35-300) antibody
CD44 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Recommended dilution: WB:1:1000-1:40000, IHC:1:50-1:200, IF:1:50-1:200,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

CD44

别名

CD44

背景

Background: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9, the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity).
Aliases: CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4

UniProt

P16070

途径

Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling