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GFP 抗体

Name: GFP 抗体
Brand: antibodies-online
SKU: ABIN7141875
Availability: OutOfStock

This anti-GFP antibody is a 小鼠单克隆 antibody detecting GFP in WB, ELISA, IP, IF 和 FACS. Suitable for Aequorea victoria.

Western Blot Positive WB detected in: 50 ng recombinant protein All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5 min (GFP 抗体)

Immunofluorescence staining of 293F cells transfected with GFP with ABIN7141875 at 1:100, counter-stained with DAPI. (GFP 抗体)

Western Blot Positive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50 ng, 5, 293F whole cell lysate transfected with GFP for 5 μg All lanes GFP antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively Observed band size: 32,32,50,32,32 KDa Exposure time:1 min (GFP 抗体)

Overlay histogram showing 293F cells transfected with GFP stained with ABIN7141875 (red line) at 1:200. (GFP 抗体)

FACS

Western Blot Positive WB detected in: Recombinant protein at 25 ng, 12. (GFP 抗体)

Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with ABIN7141875 at 1:200. (GFP 抗体)

FACS

Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP Lane 1: Mouse control IgG2b instead of ABIN7141875 in 293F whole cell lysate transfected with GFP Lane 2: ABIN7141875 (4 μg) + 293F whole cell lysate transfected with GFP (500 μg) Lane 3: 293F whole cell lysate transfected with GFP (5 μg) For western blotting, the blot was detected with ABIN7141875 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 (GFP 抗体)

7 images

产品编号 ABIN7141875

发货至: 中国

Datasheet as PDF

Quick Overview for GFP 抗体 (ABIN7141875)

抗原

See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))

适用

Aequorea victoria

宿主

小鼠

克隆类型

单克隆

标记

This GFP antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

6C11C11

Discover our top product GFP Primary Antibody

纯化方法

Protein G purified

免疫原

Recombinant GFP Protein

亚型

IgG2b
anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)
GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody (FITC)
GFP 适用: Aequorea victoria WB, IHC, FM, DB, FLISA 宿主: 山羊 Polyclonal FITC

应用备注

Recommended dilution: WB:1:50000-1:6400000, IF:1:50-1:200, FC:1:100-1:300, IP:1 μL-2 μL,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP

背景

Background: The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.
Aliases: CFP, eGFP, eYFP, GFP, GFP tag, YFP