LIM Domain Kinase 1 抗体 (pThr508)
Quick Overview for LIM Domain Kinase 1 抗体 (pThr508) (ABIN7138862)
抗原
See all LIM Domain Kinase 1 (LIMK1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr508
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
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免疫原
- Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1.
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亚型
- IgG
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应用备注
- WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- LIM Domain Kinase 1 (LIMK1)
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别名
- LIMK1
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背景
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Background: Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.
Soosairajah J, et al. (2005) EMBO J.
Ohashi K, et al. (2000) J Biol Chem, 275(5): 3577-82.
Edwards DC, et al. (1999) Nat Cell Biol, 1(5): 253-9.Aliases: EC 2.7.11.1 antibody, LIM domain kinase 1 antibody, LIM motif-containing protein kinase antibody, LIMK antibody, LIMK-1 antibody, limk1 antibody, LIMK1_HUMAN antibody
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UniProt
- P53667
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途径
- Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
抗原
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