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His Tag 抗体

This 小鼠单克隆 anti-His Tag antibody (Clone 3G5H8) specifically detects His Tag in WB 和 ELISA. The antibody is reactive with not_set samples.

Western Blot Positive WB detected in: HIS fusion protein 1 at 20 ng, 10 ng, 5 ng, 2. (His Tag 抗体)

Western Blot Positive WB detected in: HIS fusion protein 2 at 20 ng, 10 ng, 5 ng, 2. (His Tag 抗体)

2 images

产品编号 ABIN7131650

发货至: 中国

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北京 101111

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Quick Overview for His Tag 抗体 (ABIN7131650)

抗原

See all His Tag 抗体

His Tag

适用

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宿主

128
89
12
10
9
8
2
1

小鼠

克隆类型

单克隆

标记

This His Tag antibody is un-conjugated

应用范围

198
136
49
38
30
29
28
20
15
13
13
13
7
6
5
4
4
4
3
2
1
1
1
1
1
1
1

Western Blotting (WB), ELISA

克隆位点

3G5H8

Discover our top product His Tag Primary Antibody

纯化方法

Protein A purified

免疫原

HHHHHH(6x His) synthetic peptide conjugate to KLH

亚型

IgG2a
anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649

anti-His Tag antibody (HRP)
WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP

anti-His Tag antibody (Cy3)
WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3

anti-His Tag antibody (DyLight 800)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800

应用备注

Recommended dilution: WB:1:500-1:2000,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

His Tag

别名

6*His

物质类

Tag

背景

Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag

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