Recombinant CDC20 抗体
Quick Overview for Recombinant CDC20 抗体 (ABIN7127236)
抗原
See all CDC20 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human Cdc20 protein
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亚型
- IgG1
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anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibody
CDC20 适用: 人 WB, IF, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibodyCDC20 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84) antibodyCDC20 适用: 人 WB, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal AR12 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠, Pig, 马, 豚鼠, 猴, Cow, 非洲爪蟾, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-499) antibodyCDC20 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84), (N-Term) antibodyCDC20 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB23122 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠, Pig, 犬, 马, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Ramos or HeLa cells.Human tonsil or gastric carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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别名
- CDC20
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背景
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CDC20, CDC20A, p55CDC, P55CDC-LSB,Cdc20 (Cell Division Cycle Protein 20)
Cellular localisation: Cytoplasm. -
分子量
- 55kDa
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基因ID
- 991, 524947
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UniProt
- Q12834
抗原
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