Recombinant p63 抗体 (N-Term)
Quick Overview for Recombinant p63 抗体 (N-Term) (ABIN7127108)
抗原
See all p63 (TP63) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- P63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. P40/3980R recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A synthetic peptide from the N-terminal of human p40protein (Exact sequence is proprietary)
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亚型
- IgG
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anti-Tumor Protein P63 (TP63) antibody
KO Validated TP63 适用: 人 WB, ICC, IF, IP, IHC (p), IHC (fro), PLA 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P63 (TP63) (AA 421-680) antibodyTP63 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P63 (TP63) (AA 5-17) antibodyTP63 适用: 人, 小鼠, 大鼠, Cow WB, IHC, StM 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P63 (TP63) antibodyTP63 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal TP63-2428 unconjugated
anti-Tumor Protein P63 (TP63) (AA 3-106) antibodyTP63 适用: 人 WB, ELISA, Coat 宿主: 小鼠 Monoclonal TP63-1786 unconjugated
anti-Tumor Protein P63 (TP63) (AA 664-680), (C-Term) antibodyTP63 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P63 (TP63) antibodyTP63 适用: 人, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal TP63-11 unconjugated
anti-Tumor Protein P63 (TP63) (C-Term) antibodyTP63 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P63 (TP63) antibodyTP63 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P63 (TP63) (pSer455) antibodyTP63 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Human prostate or lung tissue (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- p63 (TP63) (Tumor Protein P63 (TP63))
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别名
- TP63
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背景
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DeltaNp63, TP63,p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker)
Cellular localisation: Nuclear -
分子量
- 40kDa
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基因ID
- 8626, 137569
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UniProt
- Q9H3D4
抗原
-
