ube3a 抗体
Quick Overview for ube3a 抗体 (ABIN7127039)
抗原
See all ube3a 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- E6-associating protein (E6-AP), also designated ubiquitin protein ligase E3A(UBE3A), is a component of the ubiquitin-mediated proteolytic pathway thatselectively targets proteins for degradation by the 26S Proteasome. Ubiquitin(Ub) is directly conjugated to protein substrates by the transfer of Ub from anE2 ubiquitin conjugating enzyme to the target protein. This conjugation is facil-itated by the enzymatic activity of E3 ubiquitin ligase family members such asE6-AP. Several substrates of E6-AP have been identified and include the tumorsuppressor protein p53 and the mammalian homolog of Rad23, HHR23A.Previous studies have indicated that E6-AP associates with the humanpapillomavirus E6 oncogene, which forms a complex with p53 and therebypotentiates E6-AP mediated ubiquitination of p53. Genetic mutations thatimpair E6-AP activity result in the accumulation of p53 in the cytoplasm, andin many instances, these mutations are associated with the development ofthe rare neurodevelopmental disorder Angelman syndrome (AS), which ischaracterized by severe motor dysfunction and mental retardation.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human UBE3A protein
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亚型
- IgG1
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anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 51-150) antibody
ube3a 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) antibodyube3a 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 836-865), (C-Term) antibodyube3a 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB4440 unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) antibodyube3a 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 300-590) antibodyube3a 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (Middle Region) antibodyube3a 适用: 人, 小鼠, 大鼠, 兔, 犬, 豚鼠, 马, 斑马鱼, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 83-234) antibodyube3a 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) antibodyube3a 适用: 人 IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 158-407) antibodyube3a 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Protein Ligase E3A (ube3a) (AA 180-229) antibodyube3a 适用: 人, 大鼠, 斑马鱼, 猴, Bat, Drosophila melanogaster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
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别名
- UBE3A
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背景
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ANCR, Angelman syndrome (AS), CTCL tumor antigen se37-2, E6AP ubiquitin protein ligase, EPVE6AP, FLJ26981, HECT-type ubiquitin transferase E3A, Human papilloma virus E6 associated protein Angelman syndrome, HPVE6A, NY REN 54 antigen, Oncogenic protein associated protein E6AP, Renal carcinoma antigen NY REN 54,UBE3A / E6-AP
Cellular localisation: Nucleus. Cytoplasm. -
分子量
- 100kDa
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基因ID
- 7337, 598862
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UniProt
- Q05086
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
-
