Recombinant Topoisomerase II alpha 抗体 (AA 1352-1493)
Quick Overview for Recombinant Topoisomerase II alpha 抗体 (AA 1352-1493) (ABIN7127009)
抗原
See all Topoisomerase II alpha (TOP2A) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1352-1493
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特异性
- It recognizes a 170 kDa protein, which is identified as topoisomerase IIi±. It shows no cross-reaction with Topoisomerase II i² or I. Topo II i± plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II i± is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
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交叉反应 （详细）
- Human. Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
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亚型
- IgG2a
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anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) antibody
TOP2A 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1435-1531) antibodyTOP2A 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E2 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1352-1493) antibodyTOP2A 适用: 人, 小鼠 WB, IHC, IF, StM 宿主: 小鼠 Monoclonal TOP2A-1361 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1100-1530) antibodyTOP2A 适用: 人, 大鼠 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6C12G12 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1100-1530) antibodyTOP2A 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6D4B2 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (Internal Region) antibodyTOP2A 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (N-Term) antibodyTOP2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (C-Term) antibodyTOP2A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1167-1196), (C-Term) antibodyTOP2A 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB24346 unconjugated
anti-Topoisomerase (DNA) II alpha 170kDa (TOP2A) (Thr1343) antibodyTOP2A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or Jurkat cells. Human lymph node or tonsil.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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别名
- TOP2A
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背景
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ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A,Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)
Cellular localisation: Nuclear -
分子量
- 170kDa
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基因ID
- 7153, 156346
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UniProt
- P11388
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
抗原
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