SOX4 抗体 (AA 45-130)
Quick Overview for SOX4 抗体 (AA 45-130) (ABIN7126957)
抗原
See all SOX4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 45-130
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特异性
- SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF-i²-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival invitro and for primary tumor growth and metastasis invivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.
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交叉反应 （详细）
- Human. Predicted to react in Xenopus and Chick.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa45-130) of human SOX4 protein
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亚型
- IgG2b
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anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 54-103) antibody
SOX4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (N-Term) antibodySOX4 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 斑马鱼, 山羊, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 86-114) antibodySOX4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) antibodySOX4 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal SOX4-2540 unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 86-114), (N-Term) antibodySOX4 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB18502 unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (N-Term) antibodySOX4 适用: 人, 小鼠, 大鼠, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 1-60) antibodySOX4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 45-136) antibodySOX4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) (AA 1-100) antibodySOX4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SRY (Sex Determining Region Y)-Box 4 (SOX4) antibodySOX4 适用: 人 ICC, DB 宿主: 小鼠 Monoclonal 154C4a unconjugated
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应用备注
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Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Colon or breast carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))
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别名
- SOX4
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背景
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SOX, SRY (sex determining region Y) box 4, SRY box containing gene 4, SRY related HMG box gene 4, Transcription factor SOX-4,SOX4 (Master Regulator of Epithelial-Mesenchymal Transition)
Cellular localisation: Nucleus. -
分子量
- 47kDa
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基因ID
- 6659, 643910
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UniProt
- Q06945
抗原
-
