beta Actin 抗体
Quick Overview for beta Actin 抗体 (ABIN7126900)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- All eukaryotic cells express Actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas i²-Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
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交叉反应 （详细）
- Human, Mouse and Rat. Predicted to react in Xenopus, Bovine, Chicken, Horse, Monkey, Pig and Sheep.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human ACTB protein
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亚型
- IgG1
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anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 2-16) antibodyACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 350-375) antibodyACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 1-25) antibodyACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated
anti-Actin, beta (ACTB) (AA 1-375) antibodyACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated
anti-Actin, beta (ACTB) (N-Term) antibodyKO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated
anti-Actin, beta (ACTB) (AA 1-50) antibodyACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated
anti-Actin, beta (ACTB) (N-Term) antibodyACTB 适用: 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Pig, Cow, 绵羊, Hamster, 马, 豚鼠, 山羊, Cat, Avian, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human myoepithelial cells. HeLa, Jurkat or Raji cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- ACTB
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背景
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A26C1A, A26C1B, ACTB, Actin beta, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, Melanoma X actin, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1,Beta-Actin (ACTB)
Cellular localisation: Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
分子量
- 43kDa
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基因ID
- 60, 708120
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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