RAC1 抗体
Quick Overview for RAC1 抗体 (ABIN7126866)
抗原
See all RAC1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Rac1 has been demonstrated to play a critical role in tumor progression of human colorectal adenocarcinoma cells. In one study, overexpression of Rac1 accelerated the tumorigenic process, whereas Rac1 inhibition completed suppressed tumor formation.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-length RAC1protein
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亚型
- IgG1
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anti-RAC1 (Center) antibody
适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAC1 (AA 1-192) antibody适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAC1 antibody适用: 人, 大鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1301CT276-121-104 unconjugated
anti-RAC1 (AA 11-185) antibody适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-RAC1 antibody适用: 人, 大鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 1301CT276-121-104 unconjugated
anti-RAC1 (pSer71) antibody适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAC1 (AA 101-192) antibody适用: 人, 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RAC1 antibody适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 13C4 unconjugated
anti-RAC1 (AA 49-78) antibody适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB11123 unconjugated
anti-RAC1 antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or Jurkat cells. Human breast, prostate or tonsil tissues.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- RAC1
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背景
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Cell migration-inducing gene 5 protein, MIG5, Rac-1, Ras-like protein TC25, Ras-related C3 botulinum toxin substrate 1,Ras-related C3 botulinum toxin substrate 1
Cellular localisation: Cell surface. Melanosome. -
分子量
- 21kDa
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基因ID
- 5879, 413812
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UniProt
- P63000
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途径
- WNT signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling
抗原
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