Recombinant CD31 抗体 (AA 28-330)
Quick Overview for Recombinant CD31 抗体 (AA 28-330) (ABIN7126783)
抗原
See all CD31 (PECAM1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 28-330
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特异性
- CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
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交叉反应 (详细)
- Human. Weakly reacts with Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (aa 28-330) of human CD31 expressed in E. Coli (exact sequence is proprietary)
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亚型
- IgG
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: THP-1 or Jurkat cells. Human tonsil or angiosarcoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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别名
- PECAM1
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背景
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EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic -
分子量
- ~100kDa (endothelium) and ~130kDa (platelets)
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基因ID
- 5175
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UniProt
- P16284
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途径
- Regulation of Actin Filament Polymerization
抗原
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