Recombinant CD31 抗体 (AA 28-330)
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- 抗原 See all CD31 (PECAM1) 抗体
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- 抗体类型
- Recombinant Antibody
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抗原表位
- AA 28-330
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD31 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
- 交叉反应 （详细）
- Human. Others not known.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant fragment (aa 28-330) of human CD31 expressed in E. Coli (exact sequence is proprietary)
- 亚型
- IgG1
- Top Product
- Discover our top product PECAM1 Primary Antibody
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anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 601-680) antibody
PECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, 犬 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC (p), IHC (f) 宿主: 山羊 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-382) antibodyPECAM1 适用: 人, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 681-738) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow FACS, WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-315) antibodyPECAM1 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 9E6E12 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 29-738) antibodyPECAM1 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D2-1A5 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 兔, Cynomolgus FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal JC-70A unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠, 兔, Cynomolgus FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal C31-7 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 625-738) antibodyPECAM1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal PECAM1-3527 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal C31-3 unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells), Western Blotting (1-2 μg/mL),Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- 别名
- PECAM1 (PECAM1 产品)
- 背景
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EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell surface and cytoplasm of endothelial cells - 分子量
- ~100kDa (endothelium) and ~130kDa (platelets)
- 基因ID
- 5175
- UniProt
- P16284
- 途径
- Regulation of Actin Filament Polymerization
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