PDGFB 抗体 (AA 27-158)
Quick Overview for PDGFB 抗体 (AA 27-158) (ABIN7126776)
抗原
See all PDGFB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 27-158
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特异性
- PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, AB and BB. The three isoforms bind, with different affinities, to two receptor types, i±and i², which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to i± receptors with high affinity, whereas the B subunit can bind to both i± and i² receptors. Evidence suggests that PDGF may function as a neurotrophic factor. Receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons. These facts suggest that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system through paracrine and autocrine routes.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment of human PDGFB protein (around aa 27-158) (exact sequence is proprietary)
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亚型
- IgG2
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anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 20-241) antibody
PDGFB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 155-210) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 84-199) antibodyPDGFB 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (C-Term) antibodyVerified PDGFB 适用: 人 ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 1-241) antibodyPDGFB 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (N-Term) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) antibodyPDGFB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (N-Term) antibodyPDGFB 适用: 人, 大鼠, 小鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 21-241) antibodyPDGFB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human colon, kidney or lymph node.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
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别名
- PDGFB
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背景
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Becaplermin, c sis, Oncogene SIS, PDGF Beta chain, PDGF2, Platelet derived growth factor 2, Platelet derived growth factor B chain, Platelet derived growth factor beta, Platelet derived growth factor beta polypeptide (oncogene SIS), Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog),PDGF beta (PDGFB)
Cellular localisation: Cytoplasm. -
分子量
- 14kDa (monomeric B chain), 31-35kDa (dimer)
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基因ID
- 5155, 1976
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UniProt
- P01127
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
抗原
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