Fibronectin 1 抗体
Quick Overview for Fibronectin 1 抗体 (ABIN7126426)
抗原
See all Fibronectin 1 (FN1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 29 kDa N-terminal fibrin- and heparin-binding domain of fibronectin. It does not cross-react with MMP-2, MMP-9, or TIMP-2. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
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交叉反应 (详细)
- Human and Pig.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- High molecular weight proteins secreted by cultivated human fibroblasts
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亚型
- IgG2a
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: SW156 cells. Prostate or Kidney.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Fibronectin 1 (FN1)
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别名
- FN1
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背景
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Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3,Fibronectin (Fibrin/Heparin-Binding Domain)
Cellular localisation: Connective tissue matrix -
分子量
- 220kDa (monomer), 440kDa (dimer)
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基因ID
- 2335, 203717
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UniProt
- P02751
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途径
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
抗原
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