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Fibronectin 1 抗体

This anti-Fibronectin 1 antibody is a 小鼠 单克隆 antibody detecting Fibronectin 1 in WB, IF, IHC (f) 和 IP. Suitable for 人.
产品编号 ABIN7126423
发货至: 中国

Quick Overview for Fibronectin 1 抗体 (ABIN7126423)

抗原

See all Fibronectin 1 (FN1) 抗体
Fibronectin 1 (FN1)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Fibronectin 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
  • 特异性

    Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease.

    交叉反应 (详细)

    Human. Monkey. Porcine. Bovine. Equine. Avian. Amphibian.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Human plasma fibronectin

    亚型

    IgG1
  • 应用备注

    Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: SW156 cells. Human kidney.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    Fibronectin 1 (FN1)

    别名

    FN1

    背景

    Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3,Fibronectin (Total)
    Cellular localisation: Connective tissue matrix

    分子量

    220kDa (monomer), 440kDa (dimer)

    基因ID

    2335, 203717

    UniProt

    P02751

    途径

    Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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