Recombinant CDKN1B 抗体
Quick Overview for Recombinant CDKN1B 抗体 (ABIN7504170)
抗原
See all CDKN1B 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- This MAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- ' induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
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交叉反应 （详细）
- Human.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant full-length human CDKN1B protein
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亚型
- IgG
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anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 150-198) antibody
CDKN1B 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibodyCDKN1B 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 4B4-E6 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyKO Validated CDKN1B 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal SX53G8 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人, 小鼠, 大鼠, 猴 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal DCS-72-F6-KIP1-769 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 1G2 unconjugated Recombinant Antibody
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibodyCDKN1B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibodyCDKN1B 适用: 小鼠 WB, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal DCS-72-F6 unconjugated
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应用备注
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Positive Control: HeLa or MCF7 cells. Human tonsil, breast, cervical or colon carcinoma.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
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有效期
- 24 months
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- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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别名
- CDKN1B
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背景
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CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4,p27Kip1 (Mitotic Inhibitor/Suppressor Protein)
Cellular localisation: Nuclear -
分子量
- 25-26kDa
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基因ID
- 1027, 238990
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UniProt
- P46527
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
抗原
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