Recombinant CD25 抗体
Quick Overview for Recombinant CD25 抗体 (ABIN7072732)
抗原
See all CD25 (IL2RA) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-CD25 [2E4], Mouse IgG1, kappa
-
特异性
- 2E4 recognizes mouse CD25. This epitope is distinct from the IL-2 binding site and does not block binding of IL-2 to CD25. This antibody binds the same or overlapping epitope as 7D4 (Ab02216) but different than Ab01107. Murine CD25 is a 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor.
-
产品特性
-
Original Species of Ab: Mouse
Original Format of Ab: IgG1
-
纯化方法
- Protein A affinity purified
-
免疫原
- The original version of this antibody was raised to recognize mouse CD25.
-
亚型
- IgG1 kappa
-
-
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody
IL2RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B5D12 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibodyIL2RA 适用: 人 WB, ELISA, ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibodyIL2RA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibodyIL2RA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IL2RA-2395 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 PerCP
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-235) antibodyIL2RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- 2E4 is a non-IL-2 blocking anti-CD25 antibody. The variable fragment (Fv) of this antibody conjugated to a 38- kDa portion of Pseudomonas exotoxin A exhibited exceptional ability to induce tumor rejection and lasting antitumor immunitiy in mice making it a promising canditate in the immunotherapy research and developemnt (Onda et al., 2019, pmid: 30760587). This 2E4 Fv-based CD25-specific recombinant immunotoxin (RIT) was injected into three different mouse tumor models and brought about complete regressions of the majority of them leadinig, in many cases, to immunity against tumors' reinjections and cross-tumor type immunity (Onda et al., 2019, pmid: 30760587). Finally, it was demonstrated that this immunotoxin (RIT) caused significant depletion of regulatory T cells (Tregs) in the tumor environment but, importantly, not in the spleen, at the same time, the number of effector, cytotoxic CD8 cells in the tumor increased what would suggest the possible antitumour mechanism of this immunotoxin (Onda et al., 2019, pmid: 30760587). Additionally, 2E4 can be used in flow cytometric analyses, for instance, it has been used to perform phenotyping of lymphocytes as a part of a wider panel of antibodies (Alexander et al., 2008, pmid: 18824594).
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- PBS with 0.02 % Proclin 300.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
-
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
-
别名
- CD25
-
背景
- IL-2-RA, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2R subunit alpha, IL2-RA, p55
-
UniProt
- P01590
-
途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原
-
