Recombinant PD-1 抗体 (Extracellular Domain)
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Quick Overview for Recombinant PD-1 抗体 (Extracellular Domain) (ABIN7072592)
抗原
See all PD-1 (PDCD1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- Anti-PD-1 [J43], Rabbit IgG, lambda
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特异性
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This antibody is specific for murine PD-1.
Application Notes original Clone: The specificity of this antibody has been confirmed in ELISA analysis, using PD-1 extracellular domain fusion proteins (Agata et al, 1996). Additionally, in flow cytometric analysis, this antibody reacts with PD-1 cDNA-transfected BHK and CHO cells, but not with parental BHK and CHO cells, as well as reacting with lymphocytes from PD-1 cDNA transgenic mice (Agata et al, 1996). This antibody has been used to immunoprecipitate PD-1 from lysates of PD-1 cDNA-transfected BHK and CHO cells (Agata et al, 1996), in flow cytometric quantification of CD4+PD-1+ T cells in murine spleens (Kasagi et al, 2010), and in immunohistochemical analysis of acetone-fixed murine spinal cord and brain tissue sections (Salama et al, 2003). This antibody displays diverse effects in different mouse models of disease. When administered to NZB/W F1 mice, a model of lupus-like nephritis, this antibody has been shown to delay the onset of nephritis and prolong survival, through the depletion of PD-1+ T cells (Kasagi et al, 2010). Antibody-treated NZB/W F1 mice displayed decreased numbers of PD-1+ T cells, and this antibody was confirmed to trigger complement-dependent cytotoxicity in PD-1+ T cells in vitro (Kasagi et al, 2010). Conversely, administration to experimental allergic encephalitis (EAE) and NOD diabetes mice exacerbated disease, through its neutralising activity (Salama et al, 2003, Ansari et al, 2003), this antibody has been shown in vitro to inhibit binding of both PD-L1-Ig and PD-L2-Ig to PD-1 transfected BHK cells (Ansari et al, 2003).
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产品特性
- This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.
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亚型
- IgG, lambda
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anti-Programmed Cell Death 1 (PDCD1) (AA 201-288) antibody
PDCD1 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 41-132) antibodyPDCD1 适用: 人 IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 192-288) antibodyPDCD1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8G5B12 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 192-288) antibodyPDCD1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3B3D9 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 84-117), (N-Term) antibodyPDCD1 适用: 小鼠, 大鼠 FACS, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibodyPDCD1 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3E12A10 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibodyPDCD1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 1D6F6 unconjugated
anti-Programmed Cell Death 1 (PDCD1) antibodyPDCD1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal PDCD1-2720 unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 21-170) antibodyPDCD1 适用: 人 IHC, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 (PDCD1) (AA 1-288) antibodyPDCD1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Hamster, Armenian
OriginalFormat: IgG
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
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别名
- PD-1
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背景
- CD279, PD1, PD 1, Programmed cell death protein 1, Programmed cell death 1, pdcd1, Protein PD-1, mPD-1
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UniProt
- Q02242
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途径
- Cancer Immune Checkpoints
抗原
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