Recombinant CD40 Ligand 抗体
Quick Overview for Recombinant CD40 Ligand 抗体 (ABIN7072506)
抗原
See all CD40 Ligand (CD40LG) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD40L [MR1], Rabbit IgG, kappa
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特异性
- This antibody is specific for murine CD40 ligand (CD40L), a 39 kDa transmembrane glycoprotein. CD40L is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD40L-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.
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产品特性
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Original Species of Ab: Hamster, Armenian
Original Format of Ab: IgG
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunising Armenian hamsters with murine activated Th1 (D1.6) plasma membrane as described by Noelle et al (1992).
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亚型
- IgG kappa
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anti-CD40 Ligand (CD40LG) (AA 1-261) antibody
CD40LG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 108-261) antibodyCD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2763 unconjugated
anti-CD40 Ligand (CD40LG) (AA 108-261) antibodyCD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2761 unconjugated
anti-CD40 Ligand (CD40LG) antibody (FITC)CD40LG 适用: 小鼠 FACS 宿主: 仓鼠 Monoclonal MR-1 FITC
anti-CD40 Ligand (CD40LG) (AA 79-129) antibodyCD40LG 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 33-62) antibodyCD40LG 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) (AA 111-261) antibodyCD40LG 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 Ligand (CD40LG) antibody (PE)CD40LG 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 24-31 PE
anti-CD40 Ligand (CD40LG) (AA 47-261) antibodyCD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8H10F5 unconjugated
anti-CD40 Ligand (CD40LG) (AA 47-261) antibodyCD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5A3A9 unconjugated
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应用备注
- [Yesterday 2:23 PM] Victor Koolma This antibody was administred to SOD1 G93A mice, which exhibit ALS like symptoms, it was able to delay paralysis onset, it improved body-weight maintenance and extended survival (Lincecum et al., 2010, PMID:20348957). This antibody has been used in various FACS analyses for diverse immunological applications, such as to indicate how naive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival (Lesley et al, 2006), to prove that the interaction between natural killer cells and dendritic cells has a pivotal role in the sensitization phase of contact hypersensitivity (Shimizuhira et al, 2014), and to demonstrate that enhanced CD8 T cell responses through GITR-mediated costimulation could resolve chronic viral infection (Pascutti et al, 2015). This antibody has also been used for in vitro and in vivo blocking and functional studies, for instance, to indicate that the 39- kDa CD40L membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-dependent B-cell activation (Noelle et al, 1992), and to demonstrate that the CD40L-CD40 pathway can augment the survival of autoantigen-engaged B cells in the absence of T cell activation (Lesley et al, 2006). Furthermore, this antibody clone (MR1) has been re-formatted as a rat IgG2b version and used for in vivo functional assays to suggest that short pulses of anti-CD40L antibody therapy may still be useful in tolerance protocols even when the Fc region is disabled (Daley et al, 2008).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD40 Ligand (CD40LG)
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别名
- CD40L
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背景
- CD154, CD40 ligand, CD40-L, Cd40l, gp39, HIGM1, IGM, IMD3, Ly-62, Ly62, RP23-153G22.3, T-BAM, T-cell antigen Gp39, TNF-related activation protein, Tnfsf5, TRAP, Tumor necrosis factor ligand superfamily member 5.
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UniProt
- P27548
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途径
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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