Recombinant TIM3 抗体
Quick Overview for Recombinant TIM3 抗体 (ABIN7072497)
抗原
See all TIM3 (TIM 3) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Tim-3 [2C12], Rabbit IgG, kappa
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特异性
- This antibody is specific for murine TIM-3, a Th1-specific cell surface protein. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. This antibody binds to the BALB/c allele of TIM-3 while reactivity to the C57Bl/6 allele is significantly weaker.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund's adjuvant.
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亚型
- IgG kappa
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anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (Internal Region) antibody
Verified TIM 3 适用: 人 FACS, WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (AA 22-202) antibodyTIM 3 适用: 人 ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal TIM3-3113 unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (AA 167-194) antibodyTIM 3 适用: 人 WB 宿主: 兔 Polyclonal RB41963 unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (AA 22-202) antibodyTIM 3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (AA 22-202) antibodyTIM 3 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 7C8A7 unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (AA 224-301) antibodyTIM 3 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2A7A6 unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) antibodyTIM 3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) antibodyTIM 3 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-366R unconjugated Recombinant Antibody
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (AA 22-202) antibodyTIM 3 适用: 人 FACS, WB 宿主: 兔 Polyclonal unconjugated
anti-Hepatitis A Virus Cellular Receptor 2 (TIM 3) (AA 22-202) antibodyTIM 3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
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应用备注
- This antibody was used in FACS, immunohistochemistry, immunofluorescence and a murine experimental autoimmune encephalomyelitis model to study the effects of TIM-3 triggering in dendritic cells (Anderson et al., 2007, PMID: 18006747). Furthermore, this antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al., 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al., 2010, PMID: 20819927), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al., 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al., 2005). In addition, employing this anti-TIM-3 antibody as part of the combination immuntherapy (anti-TIM3 + anti-PD-1 +/- anti-CTLA-4) has been shown to be effective against experimental and carcinogen-induced tumors in mice (Sakuishi et al., 2010, PMID: 20819927) (Ngiow et al., 2011, PMID: 21430066).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))
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别名
- Tim-3
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物质类
- Virus
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背景
- CD366, HAVcr-2, HAVCR2, Hepatitis A virus cellular receptor 2 homolog, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIMD-3, TIM3, 8B.2C12
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UniProt
- Q8VIM0
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途径
- Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints
抗原
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