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Recombinant Rhodopsin 抗体

This anti-Rhodopsin antibody is a 小鼠 Chimeric antibody detecting Rhodopsin in WB, IHC, ELISA, IP, IF 和 Lbl. Suitable for 人, Cow, 大鼠, 小鼠, 斑马鱼 和 Amphibian.
产品编号 ABIN7072289
发货至: 中国

Quick Overview for Recombinant Rhodopsin 抗体 (ABIN7072289)

抗原

See all Rhodopsin (RHO) 抗体
Rhodopsin (RHO)

抗体类型

Recombinant Antibody

适用

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人, Cow, 大鼠, 小鼠, 斑马鱼, Amphibian

宿主

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小鼠

克隆类型

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Chimeric

标记

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This Rhodopsin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Labeling (Lbl)

克隆位点

Rho 1D4
  • 原理

    Anti-Rhodopsin [Rho 1D4], Mouse IgG1, kappa

    特异性

    The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

    交叉反应

    Amphibian, Cow, 人, 大鼠, 斑马鱼

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG1

    纯化方法

    Protein A affinity purified

    免疫原

    Bleached bovine rod outer segment (ROS).

    亚型

    IgG1 kappa
  • 应用备注

    The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

    说明

    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    Rhodopsin (RHO)

    别名

    Rhodopsin

    物质类

    Chemical

    背景

    Rho-tag, Visual Purple

    UniProt

    P08100

    途径

    WNT signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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