Recombinant Ly6g 抗体
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Quick Overview for Recombinant Ly6g 抗体 (ABIN7072246)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Ly6G [1A8], Rabbit IgG, kappa
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特异性
- Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
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亚型
- IgG kappa
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anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p), Func 宿主: 大鼠 Monoclonal RB6-8C5 unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1A8 FITC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE-Cy7)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠 IHC 宿主: 大鼠 Monoclonal 1A8 unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (Biotin)Ly6g 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal RB6-8C5 Biotin
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (APC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1A8 APC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PerCP-Cy5.5)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1A8 PerCP-Cy5.5
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应用备注
- Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion < a href="http://www.ncbi.nlm.nih.gov/pubmed/17884993" target="_blank"> in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) < a href="http://absoluteantibody.com/ab/anti-f480-cia3-1-recombinant-version/" target="_blank"> (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022, PMID: 36359801).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
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别名
- Ly6G
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背景
- LGR-1 , y-6G, GR1, Lymphocyte antigen 6G
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UniProt
- P35461
抗原
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