Ly6g 抗体
(8 references)-
- 抗原 See all Ly6g products
- Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Ly6g antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
- 特异性
- The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.
- 交叉反应 （详细）
- Mouse
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 内毒素水平
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
- 免疫原
- Murine granulocytes
- 克隆位点
- RB6-8C5
- 亚型
- IgG2b
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anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (APC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE-Cy7)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1A8 FITC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (Biotin)Ly6g 适用: 小鼠 FACS, IP, WB, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal RB6-8C5 Biotin
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (violetFluor™ 450)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 violetFluor™ 450
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- 应用备注
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Functional application: Neutrophil depletion.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein.
Western blotting: Recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4
- 储存液
- Azide free
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways." in: European journal of immunology, Vol. 39, Issue 8, pp. 2281-92, (2009) (PubMed).
: "Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production." in: Experimental parasitology, Vol. 119, Issue 3, pp. 403-10, (2008) (PubMed).
: "Neutrophils are essential for rapid clearance of Enterococcus faecium in mice." in: Infection and immunity, Vol. 77, Issue 1, pp. 485-91, (2008) (PubMed).
: "Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 10, pp. 6573-80, (2007) (PubMed).
: "The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection." in: BMC immunology, Vol. 7, pp. 19, (2006) (PubMed).
: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, (2004) (PubMed).
: "Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 893-901, (2003) (PubMed).
: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).
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: "MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways." in: European journal of immunology, Vol. 39, Issue 8, pp. 2281-92, (2009) (PubMed).
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- 抗原
- Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
- 别名
- Ly6G (Ly6g 产品)
- 别名
- Gr-1 antibody, Gr1 antibody, Ly-6G antibody, lymphocyte antigen 6 complex, locus G antibody, Ly6g antibody
- 背景
- Lymphocyte antigen 6 complex, locus G,Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.,Gr1, Gr-1, Ly-6G
- 基因ID
- 546644
- UniProt
- A0A087WQF5
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