LPAR3 抗体 (Extracellular)
Quick Overview for LPAR3 抗体 (Extracellular) (ABIN7043314)
抗原
See all LPAR3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 16-29, Extracellular
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原理
- A Rabbit Polyclonal Antibody to LPA Receptor 3 (EDG7)
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 12,14 amino acid residues identical
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产品特性
- Anti-LPAR3 (EDG7) (extracellular) Antibody (ABIN7043314, ABIN7044570 and ABIN7044571) is a highly specific antibody directed against an epitope to the rat LPA3 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA3 receptor from mouse, rat, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor
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亚型
- IgG
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anti-Lysophosphatidic Acid Receptor 3 (LPAR3) antibody
LPAR3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (Internal Region) antibodyLPAR3 适用: 人, 猴 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (AA 101-200) antibodyLPAR3 适用: 人, 小鼠 ELISA, FACS, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (C-Term) antibodyLPAR3 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) antibodyLPAR3 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (AA 1-12) antibodyLPAR3 适用: 小鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (Cytoplasmic Domain) antibodyLPAR3 适用: 人, 小鼠, 大鼠, 猴, Bat, Cow, 犬, 马, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (Internal Region) antibodyLPAR3 适用: 人, 小鼠, 大鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (AA 312-330) antibodyLPAR3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 3 (LPAR3) (AA 111-160) antibodyLPAR3 适用: 人, 小鼠, 大鼠 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235904)
Blocking Peptide: (ABIN7235904)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))
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别名
- LPAR3
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背景
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Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32,Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptors have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2. All six receptors are type I, rhodopsin-like G-protein coupled receptors (GPCRs) that differ in their tissue distribution and downstream signaling pathways3.GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.Expression of LPA3 receptor has been observed in human heart, testis, prostate, pancreas, lung, ovary, and brain5 and is most abundant in mouse testis, kidney, lung, small intestine, heart, stomach, spleen, brain, and thymus6.LPA3 receptor null mice are viable and grossly normal, but female nulls show a striking phenotype in the reproductive system7. However, despite the fact that LPA3 is expressed in the frontal cortex, hippocampus, and amygdala, no phenotypes related to LPA3 loss in the nervous system have been reported to date5.
Alternative names: LPAR3 (EDG7), Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32 -
基因ID
- 66025
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NCBI登录号
- NM_012152
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UniProt
- Q8K5E0
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途径
- Regulation of Cell Size
抗原
-
