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HRH1 抗体 (Extracellular)

Name: HRH1 抗体 (Extracellular)
SKU: ABIN7043269
Availability: OutOfStock

HRH1 适用: 小鼠 WB, IHC, IF, IF (cc), LCI 宿主: 兔 Polyclonal unconjugated

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6): - 1-3. (HRH1 抗体 (Extracellular))

Expression of Histamine H1 Receptor in mouse substantia nigra - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Histamine H1 Receptor/HRH1 Antibody (extracellular) (ABIN7043269, ABIN7044512 and ABIN7044513), (1:300), followed by goat anti-rabbit-AlexaFluor-488. (HRH1 抗体 (Extracellular))

IHC

Cell surface detection of Histamine H1 Receptor in live intact human MEG-01 megakaryoblastic leukemia cells: (black line) Cells. (HRH1 抗体 (Extracellular))

FACS

Western blot analysis of mouse brain membranes (lanes 1 and 5), rat brain membranes (lanes 2 and 6), rat lung membranes (lanes 3 and 7) and mouse lung membranes (lanes 4 and 8): - 1-4. (HRH1 抗体 (Extracellular))

4 images

产品编号 ABIN7043269

发货至: 中国

Datasheet as PDF

抗原 See all HRH1 抗体

HRH1 (Histamine Receptor H1 (HRH1))
抗原表位
16

13

7

5

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 176-191, Extracellular
适用
66

12

11

4

4

4

1
小鼠
宿主
66

3

2
兔
Compatible Secondaries
克隆类型
68

3
多克隆
标记
35

5

5

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This HRH1 antibody is un-conjugated
应用范围
46

32

19

19

15

15

14

9

4

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

原理

A Rabbit Polyclonal Antibody to Histamine H1 Receptor

特异性

2nd extracellular loop.

交叉反应

人, 小鼠, 大鼠

预测反应

Mouse, human - 14,16 amino acid residues identical,rat - 15

产品特性

Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (ABIN7043269, ABIN7044512 and ABIN7044513)) is a highly specific antibody directed against an epitope of mouse H1R. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize H1R from rat, mouse, and human samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

Immunogen: Synthetic peptide
Immunogen Sequence: (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor

亚型

IgG

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anti-Histamine Receptor H1 (HRH1) (Cytoplasmic Domain) antibody
HRH1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H1 (HRH1) (C-Term) antibody
Verified HRH1 适用: 人, 大鼠 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Histamine Receptor H1 (HRH1) (Extracellular Domain) antibody
HRH1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H1 (HRH1) (AA 312-414) antibody
HRH1 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 3D1 unconjugated

anti-Histamine Receptor H1 (HRH1) antibody
Microarray verified HRH1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB551360 unconjugated

anti-Histamine Receptor H1 (HRH1) (Internal Region) antibody
HRH1 适用: 人 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H1 (HRH1) (Internal Region) antibody
Verified HRH1 适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Histamine Receptor H1 (HRH1) (AA 396-409) antibody
HRH1 适用: 大鼠 WB, IHC, IF, IF (cc), IC 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H1 (HRH1) (AA 141-240) antibody
HRH1 适用: 人 ELISA, WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200

说明

Negative Control: BLP-HR006
Blocking Peptide: BLP-HR006

限制

仅限研究用
状态

Lyophilized

溶解方式

0.2 mL double distilled water (DDW).

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

HRH1 (Histamine Receptor H1 (HRH1))

别名

HRH1 (HRH1 产品)

背景

Histamine Receptor H1, H1R,Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.H1 receptor is excitatory and couples to Gq-type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.

Alternative names: Histamine Receptor H1, H1R

基因ID

15465

NCBI登录号

NM_000861

UniProt

P70174

途径

Regulation of Carbohydrate Metabolic Process