HRH1 抗体 (Cytoplasmic Domain)
-
- 抗原 See all HRH1 抗体
- HRH1 (Histamine Receptor H1 (HRH1))
-
抗原表位
- Cytoplasmic Domain
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This HRH1 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human HRH1 / Histamine H1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human (100%) Chimpanzee, Gorilla (95%) Orangutan, Gibbon, Monkey, Marmoset (89%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human HRH1 / Histamine H1 Receptor. Percent identity with other species by BLAST analysis: Human (100%), Chimpanzee, Gorilla (95%), Orangutan, Gibbon, Monkey, Marmoset (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product HRH1 Primary Antibody
-
anti-Histamine Receptor H1 (HRH1) (AA 211-416) antibody
HRH1 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H1 (HRH1) (C-Term) antibodyVerified HRH1 适用: 人, 大鼠 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Histamine Receptor H1 (HRH1) (2nd Extracellular Loop), (AA 176-191) antibodyHRH1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H1 (HRH1) (Extracellular Domain) antibodyHRH1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H1 (HRH1) antibodyMicroarray verified HRH1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB551360 unconjugated
anti-Histamine Receptor H1 (HRH1) (AA 312-414) antibodyHRH1 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 3D1 unconjugated
anti-Histamine Receptor H1 (HRH1) (Internal Region) antibodyHRH1 适用: 人 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H1 (HRH1) (Internal Region) antibodyVerified HRH1 适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Histamine Receptor H1 (HRH1) (3rd Intracellular Loop), (AA 396-409) antibodyHRH1 适用: 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (4 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- HRH1 (Histamine Receptor H1 (HRH1))
- 别名
- HRH1 / Histamine H1 Receptor (HRH1 产品)
- 别名
- H1-R antibody, hisH1 antibody, Bphs antibody, H1R antibody, HH1R antibody, Hir antibody, HRH1 antibody, H1 antibody, Hisr antibody, histamine receptor H1 antibody, histamine receptor H 1 antibody, HRH1 antibody, Hrh1 antibody, hrh1 antibody
- 背景
-
Name/Gene ID: HRH1
Subfamily: Histamine
Family: GPCR
Synonyms: HRH1, H1 histamine receptor, H1 receptor, Histamine 1 receptor, Histamine H1 receptor, H1-R, H1R, Histamine H(1) receptor, Histamine receptor H1, HisH1 - 基因ID
- 3269
- 途径
- Regulation of Carbohydrate Metabolic Process
-
