GPR84 抗体 (Extracellular) (FITC)
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- 抗原 See all GPR84 抗体
- GPR84 (G Protein-Coupled Receptor 84 (GPR84))
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抗原表位
- AA 166-180, Extracellular
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GPR84 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Live Cell Imaging (LCI)
- 原理
- A Rabbit Polyclonal Antibody to GPR84 Conjugated to the Fluorescent Dye FITC
- 特异性
- 2nd extracellular loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Human - 14,15 amino acid residues identical, mouse,rat - 13
- 产品特性
- Anti-GPR84 (extracellular) Antibody (ABIN7043175, ABIN7044443 and ABIN7044444)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples. \nAnti-GPR84 (extracellular)-FITC Antibody (ABIN7043175, ABIN7044443 and ABIN7044444)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84
- 亚型
- IgG
- Top Product
- Discover our top product GPR84 Primary Antibody
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
- 说明
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Negative Control: RIC-001-F
Blocking Peptide: BLP-GR052
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 15 μL or 50 μL double distilled water (DDW), depending on the sample size.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- GPR84 (G Protein-Coupled Receptor 84 (GPR84))
- 别名
- GPR84 (GPR84 产品)
- 背景
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G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33,GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.
Alternative names: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33 - 基因ID
- 53831
- NCBI登录号
- NM_020370
- UniProt
- Q9NQS5
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