GPR68 抗体 (Extracellular)
Quick Overview for GPR68 抗体 (Extracellular) (ABIN7043170)
抗原
See all GPR68 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 169-183, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR68 (OGR1)
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特异性
- 2nd extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse,human - 14,15 amino acid residues identical
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产品特性
- Anti-GPR68 (OGR1) (extracellular) Antibody (ABIN7043170, ABIN7044425 and ABIN7044426) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68
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亚型
- IgG
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anti-G Protein-Coupled Receptor 68 (GPR68) (AA 276-365) antibody
GPR68 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (C-Term) antibodyGPR68 适用: 人, 小鼠, 大鼠, 马, Cow, 兔, 猴, Pig, Hamster IHC, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (AA 276-365) antibodyGPR68 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Cytoplasmic Domain) antibodyGPR68 适用: 人, 小鼠, 大鼠, 马, Cow, 兔, 猴, 犬, Pig, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Extracellular Domain) antibodyGPR68 适用: 人, 大鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Extracellular Domain) antibodyGPR68 适用: 人, 大鼠, 猴 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Internal Region) antibodyGPR68 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) antibodyGPR68 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (AA 281-330) antibodyGPR68 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) antibodyGPR68 适用: 人 WB, IHC, InhA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: IFC
Negative Control: (ABIN7235438)
Blocking Peptide: (ABIN7235438)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR68 (G Protein-Coupled Receptor 68 (GPR68))
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别名
- GPR68
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背景
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Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor,All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.
Alternative names: GPR68/OGR1, Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor -
基因ID
- 238377
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NCBI登录号
- NM_001177676
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UniProt
- Q8BFQ3
抗原
-
