GPR68 抗体 (2nd Extracellular Loop) (APC)
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Quick Overview for GPR68 抗体 (2nd Extracellular Loop) (APC) (ABIN7582036)
抗原
See all GPR68 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- 2nd Extracellular Loop, AA 169-183
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原理
- A Rabbit Polyclonal Antibody to GPR68 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
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序列
- (C)HRV(S)FEHYPIQAWQ R
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预测反应
- Rat,mouse,human - 14,15 amino acid residues identical
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产品特性
- Anti-GPR68 (OGR1) (extracellular) Antibody (ABIN7043170, ABIN7044425 and ABIN7044426) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize GPR68 from mouse, rat and human samples. Anti-GPR68 (OGR1) (extracellular)-APC Antibody (ABIN7043170, ABIN7044425 and ABIN7044426-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68
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亚型
- IgG
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anti-G Protein-Coupled Receptor 68 (GPR68) (AA 276-365) antibody
GPR68 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (C-Term) antibodyGPR68 适用: 人, 小鼠, 大鼠, 马, Cow, 兔, 猴, Pig, Hamster IHC, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Extracellular Domain) antibodyGPR68 适用: 人, 大鼠, 猴 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Internal Region) antibodyGPR68 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Cytoplasmic Domain) antibodyGPR68 适用: 人, 小鼠, 大鼠, 马, Cow, 兔, 猴, 犬, Pig, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (Extracellular Domain) antibodyGPR68 适用: 人, 大鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (AA 276-365) antibodyGPR68 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (AA 200-225) antibodyGPR68 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) (2nd Extracellular Loop), (AA 169-183) antibodyGPR68 适用: 小鼠 WB, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 68 (GPR68) antibodyGPR68 适用: 人 WB, IHC, InhA 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: The optimal concentration should be determined by the user
FC: 5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7582043)
Blocking Peptide: (ABIN7235438)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- GPR68 (G Protein-Coupled Receptor 68 (GPR68))
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别名
- GPR68
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背景
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Synonyms: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor, AI2A6
Description: All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.
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基因ID
- 238377
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UniProt
- Q8BFQ3
抗原
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