FZD1 抗体 (Extracellular, N-Term)
Our Local Distributor
北京 101111
Quick Overview for FZD1 抗体 (Extracellular, N-Term) (ABIN7043140)
抗原
See all FZD1 (Fzd1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 238-250, Extracellular, N-Term
-
原理
- A Rabbit Polyclonal Antibody to Frizzled-1
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- Mouse,human - identical
-
产品特性
- Anti-Frizzled-1 (FZD1) (extracellular) Antibody (ABIN7043140, ABIN7044291 and ABIN7044292) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize FZD1 from rat, mouse, and human samples.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
- (C)PEFWTSNPQHGGG, corresponding to amino acid residues 238-250 of rat Frizzled-1
-
亚型
- IgG
-
-
anti-Frizzled Family Receptor 1 (Fzd1) (AA 117-322) antibody
Fzd1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (AA 73-322) antibodyFzd1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (AA 367-396) antibodyFzd1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB14325 unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (AA 374-404), (Middle Region) antibodyFzd1 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (N-Term) antibodyFzd1 适用: 人, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (N-Term) antibodyFzd1 适用: 人, 小鼠, 大鼠, Bat, Cow, 犬, Hamster, 马, 猴, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (AA 504-533), (C-Term) antibodyFzd1 适用: 人 WB 宿主: 兔 Polyclonal RB14327 unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (AA 369-400), (C-Term) antibodyFzd1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) antibodyFzd1 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 1 (Fzd1) (N-Term) antibodyFzd1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
WB: 1:200
FC: 2.5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
-
说明
-
Negative Control: (ABIN7235337)
Blocking Peptide: (ABIN7235337)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
-
浓度
- 0.8 mg/mL
-
缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
-
-
- FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))
-
别名
- FZD1
-
背景
-
Synonyms: Frizzled class receptor 1, Fz-1
Description: Frizzled class receptor 1 (also known as FZD1) belongs to the frizzled subfamily of G protein-coupled receptors. Currently, the frizzled family of GPCRs contains 10 frizzled proteins (FZD1-10) and Smoothened (SMO).Similar to other GPCRs, FZD1 consists of a single polypeptide with an extracellular N-terminus, an intracellular C-terminus, seven hydrophobic transmembrane domains (TM1-TM7) linked by three extracellular loops (ECL1-ECL3) and three intracellular loops (ICL1-ICL3). FZD1 is encoded by the firzzled-1 gene which is mainly expressed in the adult heart, placenta, lung, kidney, pancreas, prostate and ovary.FZD1 is activated by WNTs, which are cysteine-rich lipo-glycoproteins with fundamental functions in ontogeny and tissue homeostasis. Signaling through the WNT/FZD complex can lead to the activation of pertussis toxin-sensitive heterotrimeric G proteins, the elevation of intracellular calcium activation of cGMP-specific PDE6 and elevation of cAMP as well as RAC-1, JNK, Rho and Rho kinase signaling1.The relation between the FZD1 pathway and various pathologies has been researched extensively. Reduced levels of FZD1 can be found in the spinal cords of ALS transgenic mice, possibly due to the increase of FZD1 protein degradation or the inhibition of FZD1 protein expression in the spinal cord in the disease process of ALS transgenic mice2. The FZD1 signaling pathway has also been implicated in inflammatory bowel disease. Patients with ulcerative colitis exhibit reduced expression of FZD1. FZD1 is overexpressed in the multi drug resistant cancer cell subline MCF-7/ADM and seems to mediate multidrug resistance by regulating the Wnt/β-catenin pathway3.
-
基因ID
- 58868
-
NCBI登录号
- NM_003505
-
UniProt
- Q08463
-
途径
- WNT signaling, Asymmetric Protein Localization
抗原
-
